华中农业大学

地址：430070 湖北省武汉市华中农业大学园艺植物生物学教育部重点实验室

Tel: 027-87281543 (O);

Email: guoww@mail.hzau.edu.cn

郭文武简介：男，1970年9月生，湖北郧县人；博士、教授、博士生导师。1993年7月本科毕业于华中农业大学园艺系；1993-1998年，华中农业大学攻读硕士、博士学位；1998年6月毕业，获博士学位并留校任教。1999、2003年，分别晋升为副教授、教授。2001-2003年，美国佛罗里达大学柑橘研究与教育中心博士后；2007年，澳大利亚CSIRO园艺部、澳大利亚国立大学高访3个月；2009年，日本鹿儿岛大学高访2个月。

人才团队计划、科研奖励和荣誉称号：入选国家杰出青年科学基金（2011）、教育部长江学者特聘教授（2012）、国家特支计划（万人计划）首批科技创新领军人才、“园艺植物细胞工程与种质创新”教育部创新团队和湖北省创新研究群体学术带头人（2013）、教育部首批新世纪优秀人才、科技部首批中青年科技创新领军人才；曾获湖北省自然科学二等奖（排名第二，2001）、国家科技进步二等奖（排名第三，2007）、第11届中国青年科技奖（2010）。

国内外学术兼职：《Scientia Horticulturae》共同主编（2014.3—）、咨询编委（2005.3—2014.2），《Plant Cell Tissue Organ Culture》Associate Editor (2009.10—)、《Tree Genetics Genomes》Associate Editor（2011.12—）。中国柑橘学会理事（2009.10—）、中国园艺学会理事（2013.10—）。

主持科研项目：先后主持国家自然科学基金（青年、面上、国家杰青）、科技部973计划课题、863计划课题、教育部项目（创新团队发展计划、重大项目培育计划、霍英东青年教师基金、博士点基金及优先课题）、农业部公益性行业科研专项子项目、瑞典国际科学基金、湖北省自然科学基金（创新群体、重点、杰出青年）、武汉市晨光计划等。

研究兴趣与重点内容：从事柑橘种质创新与遗传改良研究。针对我国柑橘在品种、品质等方面存在的问题，以及常规育种中遇到的珠心胚干扰等生殖障碍，开展细胞工程与遗传改良研究。旨在提高育种效率、缩短育种周期，培育具有无核、优质、不同熟期等性状的新品种，充分发挥“细胞工程”这一“传统且不存在安全性争议”的生物技术在柑橘遗传改良中的独特作用。重点研究内容如下：1）体细胞胚、珠心胚发育的分子机理；2）细胞工程与种质创新：体细胞杂种、胞质杂种创制，三倍体创制，同源多倍体发掘与利用，纯系创制，细胞工程新品种选育；3）细胞工程新种质特殊性状解析与分子育种：胞质杂种雄性不育性状解析与基因发掘，人工合成多倍体的体细胞互作遗传、表观遗传变异分析，转基因技术与分子育种。

发表论著：已在J Proteome Res、DNA Research、BMC Genomics、PLoS One、Theor Appl Genet、Planta、Plant Cell Reports、Tree Genetics Genomes、Plant Science、Protoplasma、Genome、Plant Cell Tissue Organ Culture、Plant Breeding、Euphytica、Scientia Horticulturae、中国农业科学、园艺学报等国内外刊物发表第一和通讯作者论文60余篇。部分论著如下（* 通讯作者）：

1.　 解凯东，王惠芹，王晓培，梁武军，谢宗周，伊华林，邓秀新，Grosser JW，郭文武*（2013）单胚性二倍体为母本与异源四倍体杂交大规模创制柑橘三倍体。中国农业科学 46：4550-4557

2.　 解凯东，王晓培，王惠芹，梁武军，谢宗周，郭大勇，伊华林，邓秀新，Grosser JW，郭文武*（2014）以柑橘多胚性二倍体母本倍性杂交培育三倍体。园艺学报 41：613-620

3.　 梁武军，解凯东，郭大勇，谢宗周，徐强，伊华林，郭文武*（2014）柑橘10个品种实生后代多倍体的发掘与SSR鉴定。园艺学报 41：409-416

4.　 梁武军，解凯东，郭大勇，谢宗周，伊华林，郭文武*（2014）10个柑橘砧木类型同源四倍体的发掘与SSR鉴定。果树学报 31：1-6

5.　Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics Genomes, 10.1007/s11295-014-0780-7

6.　Xu SX, Cai DF, Tan FQ, Fang YN, Xie KD, Grosser JW, Guo WW (2014) Citrus somatic hybrid: an alternative system to studyrapid structural and epigenetic reorganization in allotetraploid genomes. Plant Cell Tissue Organ Culture, DOI: 10.1007/s11240-014-0551-z

7.　Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports, DOI: 10.1007/s00299-014-1643-2

8.　Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res 13: 2998-3015

9.　Xiao X, Ma F, Chen CL*, Guo WW* (2014)High efficient transformation of auxin reporter gene into trifoliate orangevia Agrobacterium rhizogenes-mediated co-transformation. Plant Cell Tissue Organ Culture 118: 137-146

10.Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue Organ Culture 116: 81-88

11. Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliatabasedon genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150

12.　Liu Z, Ge XX, Wu XM, Kou SJ, Chai LJ, Guo WW* (2013) Selection and validation of suitable reference genes for mRNA qRT-PCR analysis using somatic embryogenic cultures, floral and vegetative tissues in citrus. Plant Cell Tissue Organ Culture 113: 469-481

13.　Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26

14.　Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA micro. BMC Genomics 13: 397

15.　Zheng BB, Wu XM, Ge XX, Deng XX, Grosser JW, Guo WW* (2012) Comparative transcript profiling of a male sterile cybrid pummelo and its fertile type revealed altered gene expression related to floral organ development. PLoS ONE 7(8): e43758

16.　Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based micro. Planta 236: 1107-1124

17.　Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163

18.　Wen L, Tan B, Guo WW* (2012) Estimating transgene copy number in precocious trifoliate orange by TaqMan real-time PCR. Plant Cell Tissue Organ Culture 109: 363–371

19.　Fan J, Liu X, Xu SX, Xu Q, Guo WW* (2011) T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange. Plant Cell Tissue Organ Culture 107: 225-232

20.　Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540

21.　Amar MH, Biswas MK, Zhang ZW, Guo WW* (2011) Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Scientia Horticulturae 128: 220-227

22.　Fu J, Peng ZJ, Cai XD, Guo WW* (2011) Regeneration and molecular characterization of interspecific somatic hybrids between Satsuma mandarin and two seedy sweet oranges for scion improvement. Plant Breeding 130: 287-290

23.　Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta 233: 495-505

24.　Wu XM, Liu MY, Xu Q, Guo WW* (2011) Identification and characterization of microRNAs from citrus expressed sequence tags. Tree Genetics Genomes 7: 117-133

25.　Xu SX, Cai XD, Tan B, Guo WW* (2011) Comparison of expression of three different sub-cellular targeted GFPs in transgenic Valencia sweet orange by confocal laser scanning microscopy. Plant Cell Tissue Organ Culture 104: 199-207

26.　Wang L, Pan ZY, Guo WW* (2010) Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo. Plant Cell Tissue Organ Culture 103:165–174

27.　Ge XX, Fan GE, Chai LJ, Guo WW* (2010) Cloning, molecular characterization and expression analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene (CitSERK1-like)in Valencia sweet orange. Acta Physiol Plant 32:1197–1207

28.　Duan YX, Fan J, Guo WW* (2010) Regeneration and characterization of transgenic kumquat plants containing the Arabidopsis APETALA1 gene. Plant Cell Tissue Organ Culture 100: 273-281

29.　Cai XD, Duan YX, Fu J, Guo WW* (2010) Production and molecular characterization of two new citrus somatic hybrids for scion improvement. Acta Physiol Plant 32: 215–221

30.　Baig MNR, Yu A, Guo WW*, Deng XX* (2009) Construction, characterization of two Citrus BAC libraries and identification of clones containing Phytoene synthase gene. Genome 52: 484-489

31.　Cai XD, Fu J, Chen CL, Guo WW* (2009) Cybrid / hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos. Scientia Horticulturae 122: 323-327

32.　Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics Genomes 5: 529-537

33.　Guo WW*, Wu RC, Fan GE, Cheng YJ (2008) Analysis of mitochondrial genomes in Citrus interspecific somatic hybrids produced by protoplast fusion. Botanical Studies 49: 295-300

34.　Guo WW*, Wu RC, Cheng YJ, Deng XX (2008) Regeneration and molecular characterisation of two interspecific somatic hybrids for potential citrus rootstock improvement. J Hort Sci Biotech 83: 407-410

35.　Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue Organ Culture 90: 275-283

36.　Duan YX, Liu X, Fan J, Li DL, Wu RC, Guo WW* (2007) Multiple shoot induction from seedling epicotyls and transgenic citrus plant regeneration containing the green fluorescent protein gene. Botanical Studies 48: 165-171

37.　Guo WW*, Wu RC, Cheng YJ, Deng XX (2007) Production and molecular characterization of citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Breeding 126: 72-76

38.　Cai XD, Liu X, Guo WW* (2006) GFP expressionas an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage. Plant Cell Tissue Organ Culture 87: 245-253

39.　Guo WW*, Cheng YJ, Chen CL, Deng XX (2006) Molecular analysis revealed autotetraploid, diploid and tetraploid cybrid plants regenerated from an interspecific somatic fusion in Citrus. Scientia Horticulturae 108: 162-166

40.　Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545

41.　Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486

42.　Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758

43.　Guo WW, Prasad D, Serrano P, Gmitter FG Jr, Grosser JW (2004) Citrus somatic hybridization with potential for direct tetraploid scion cultivar development. J Hort Sci Biotechnol 79: 400-405

44.　Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834

45.　Guo WW, Deng XX (2001) Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement. Euphytica 118:175-183

46.　Guo WW, Deng XX, Yi HL (2000) Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding. Euphytica 116: 281-285

47.　Guo WW, Zhou CH, Yi HL, Deng XX (2000) Intergeneric somatic hybrid plants between Citrus and Poncirus trifoliata and evaluation of their root rot resistance. Acta Botanica Sinica (currently J Integ Plant Biol) 42: 668-672

48.　Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585

49.　Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300

50.　Guo WW, Deng XX, Shi YZ (1998) Optimization of electrofusion parameters and interspecific somatic hybrid regeneration in citrus. Acta Botanica Sinica(currently J Integ Plant Biol) 40: 417-424

(Updated Sep 2014)