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分类:导师信息 来源:中国考研网 2015-07-13 相关院校:华中农业大学
基本信息
姓名:金双侠 出生年月:1979.2
性别:男 硕/博导:硕导
民族:汉 开设课程:植物生物技术,作物生产实习,育种学实习
职称:副教授 研究方向:棉花生物技术;细胞质基因组及生物技术
学位:博士
联系方式办公电话:027-87283955
电子邮件:jinsx1979@gmail.com
个人简介华中农业大学植物科学技术学院作物遗传育种专业博士,副教授,硕士生导师。1997-2001,华中农业大学农学系农学专业就读;2001-2006,华中农业大学植物科学技术学院攻读硕士、博士学位,获农学博士学位,指导教师均为张献龙教授.2007.10-2012.4在美国UniversityofCentralFlorida,HenryDaniell教授指导下进行博士后研究,研究方向为植物叶绿体转化。研究领域主要涉及棉花细胞、组织培养、棉花转基因等。目前从事棉花抗病,虫及逆境基因的克隆及高效表达载体(叶绿体转化表达体系)的开发及转基因研究
科研项目湖北省自然科学基金重点项目(杰青项目)主持,2015.1-2016.12
国家自然科学基金烟粉虱与棉花互作的分子机理研究主持,2014-2017
国家自然科学基金植物凝集素基因在棉花中的功能分析,主持,2012-2015
新疆建设兵团博士资金项目转基因抗病虫、抗逆聚合杂交棉新组合选育技术研究,主持,2007-2009
国家自然科学基金棉花外源基因叶绿体转化体系的建立及其应用(30771368),主持,2008-2010
教育部博士点新教师基金以gfp基因为报告基因监测转基因棉花花粉漂移(20070504087),主持,2008-2010
国家自然科学基金重大国际合作项目Developmentofstrongpest-resistancecottonviatransformingplastidanditsapplicationinbreeding,主要参加者,2009-2011
国家自然科学基金利用基因诱捕构建棉花功能基因组研究突变群体,主要参加,2005-2007
发明专利及获奖情况获奖情况:
湖北省优秀博士学位论文(第10批)获奖者,2008年
湖北省优秀本科论文指导奖,2008年。本人指导的2007界本科生张碧瑶,其论文:新疆棉花高体细胞胚胎发生能力基因型的筛选,获得湖北省优秀本科学位论文。
“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖
获第十、十一届湖北省自然科学优秀论文一等奖2次
申报两项发明专利:
超声波辅助农杆菌转化棉花胚芽的方法,专利申请号:200710063650.0
一种分离棉花叶绿体DNA的方法,专利申请号:200710052665.7
发表的论文及著作[1]JinShuanxia,Singh,Dolendro,LiLebin,Zhang,XianlongandDaniell,Henry.EngineeredchloroplastdsRNAsilencescytochromep450monooxygenase,V-ATPaseandchitinsynthasesgenesintheinsectgutanddisruptsHelicoverpaarmigeralarvaldevelopmentandpupation.PlantBiotechnologyJournal,2015,online,doi:10.1111/pbi.12355.
[2]LebinLi§•YiZhu§•ShuangxiaJin*•XianlongZhang.PyramidingBtgenesforincreasingresistanceofcottontotwomajorlepidopteranPests:SpodopteralituraandHeliothisarmigera.ActaPhysiologiaePlantarum,2014,36(10),2717-2727(SCI,IF:1.5)
[3]ShuangxiaJinandHenryDaniell.Expressionofγ-tocopherolmethyltransferaseinchloroplastsresultsinmassiveproliferationoftheinnerenvelopemembraneanddecreasessusceptibilitytosaltandmetal-inducedoxidativestressbyreducingreactiveoxygenspecies.PlantBiotechnologyJournal,2014(12),1274-1285.(SCI,IF:6.3)
[4]KwangChulKwon*,DheerajVerma*,ShuangxiaJin*,HenryDaniell.Exportofproteinsfromchloroplastsinducedbyreactiveoxygenspeciesduringbioticandabioticstress.PLOSONE,2013,8(6):e67106.(*并列第一).(SCI,IF:3.7)
[5]DheerajVerma,ShuangxiaJin,AndersonKanagaraj,NameirakpamD.Singh1,JaiyanthDaniel1,PappachanEKolattukudy1,MichaelMiller2andHenryDaniell1*ExpressionofFungalCutinaseandSwollenininTobaccoChloroplastsRevealsNovelEnzymeFunctionsand/orSubstrates.PLOSONE,2013;8(2):e57187(SCI,IF:3.7)
[6]GuanzeLiu,XuelinLi,ShuangxiaJin,XuyanLiu,LongfuZhu,YichunNie,XianlongZhang.OverexpressionofRiceNACGeneSNAC1ImprovesDroughtandSaltTolerancebyEnhancingRootDevelopmentandReducingTranspirationRateinTransgenicCotton.2014.PLoSOne,e86895
[7]WangY,JinS,WangM,ZhuL,ZhangX(2013)IsolationandCharacterizationofaConservedDomainintheEremophyteH+-PPaseFamily.PLoSONE8(7):e70099.(SCIIF3.7)
[8]ShuangxiaJin,XianlongZhang1,HenryDaniell.Pinelliaternataagglutininexpressioninchloroplastsconfersbroadspectrumresistanceagainstaphid,whitefly,lepidopteraninsects,bacterialandviralpathogens.PlantBiotechnologyJournal2012,10(3):313-327(CoverStory,IF6.3).
[9]JinSX,liuGZ,ZhuHG,YangXYandZhangXL.TransformationofUplandCotton(GossypiumhirsutumL.)withgfpGeneasaVisualMarker.JournalofIntegrativeAgriculture,2012,11(6):910-919,(SCI)
[10]LiuG,JinS,LiuX,TanJ,YangX,ZhangX(2012)OverexpressionofArabidopsiscyclinD2;1incottonresultsinleafcurlingandotherplantarchitecturalmodifications.PlantCell,TissueOrganCult110(2):261273(SCI,IF:3.6)
[11]ShuangxiaJin,AndersonKanagaraj,DheerajVerma,TheoLange,HenryDaniell.ReleaseofHormonesfromConjugates:ChloroplastExpressionofß-glucosidaseResultsinElevatedPhytohormoneLevelsAssociatedwithSignificantIncreaseinBiomassandProtectionfromAphidsorWhitefliesConferredbySucroseEsters.PlantPhysiol,2011,155,222-235.(SCI,IF:6.5)
[12]Seung-BumLee,BaichuanLi,ShuangxiaJinandHenryDaniell.Expressionandcharacterizationof antimicrobialpeptidesRetrocyclin-101andProtegrin-1inchloroplaststopreventsexualtransmissionofdiseases.PlantBiotechnologyJournal.2011,9.100-115.(SCI,IF:6.3)
[13]VermaD,KanagarajA,JinSX,SinghN,KolattukudyP,DaniellH.Chloroplast-derivedenzymecocktailshydrolyselignocellulosicbiomassandreleasefermentablesugars.PlantBiotechnologyJournal,2010,8:332-350 (SCI,IF:6.3).
[14]JinSX,ZhangXL,ZhuHG,ZhouZL,NieYC.Detectionofsomaclonalvariationofcotton(Gossypiumhirsutum)throughRAPD,SSRmarkers,cytogeneticandflowcytometeranalysis.PlantCellReports,2008,27:1303–1316.(SCI)
[15]ShuangxiaJin,XianlongZhang,etal,Identificationofanovelelitegenotypeforinvitrocultureandgenetictransformationofcotton.BiologiaPlantarum,200650(4):519-524.(SCI)
[16]ShuangxiaJin,XianlongZhang(2006)Anefficientgraftingsystemfortransgenicplantrecoveryincotton(GossypiumhirsutumL.).PlantCell,TissueandOrganCulture,85(5):181-185.(SCI)
[17]ShuangxiaJin,XianlongZhang(2005)FactorsaffectingstabletransformationandplantregenerationduringtransformingembryogeniccallusofUplandcotton(GossypiumhirsutumL.)viaAgrobacteriumtumefaciens,PlantCell,TissueandOrganCulture,81(2):229-237.(SCI)
[18]ShuangxiaJin,XianlongZhang(2005)FunctionAnalysisofPromoterTrappingSystemafterInsertingCotton(GossypiumhirsutumL.)Genome.JGENETGENOMICS(ActaGeneticaSinca),32(12):1266-1274.
[19]XianlongZhangandShuangxiaJin(2007)TransgenicCotton:AnOverview(invitedreview).TransgenicPlantJournal,2007,1(1),150-162.
[20]FangDing,ShuangxiaJin,NiHong,YunZhong,QingCao,GanjunYiandGuopingWang.2008.Vitrification–cryopreservation,anefficientmethodforeliminatingCandidatusLiberobacterasiaticus,thecitrusHuanglongbingpathogen,frominvitroadultshoottips.PlantCellReports,27(2),241-250(SCI)
[21]SunYQ,ZhangXL,JinSX.Productionandcharacterizationofsomatichybridsbetweenuplandcotton(Gossypiumhirsutum)andwildcotton(G.klotzschianumAnderss)viaelectrofusion.TheorApplGenet,2004,109:472-479(SCI)
[22]SunYQ,ZhangXL,JinSXSomaticembryogenesisandplantregenerationinwildcotton(GossypiumklotzschianumAnderss).PlantCellTissureOrganCult,2003,75:247-253(SCI)
[23]WuJH,ZhangXL,JinSX.FactorsaffectingsomaticembryogenesisandplantregenerationfromarangeofrecalcitrantgenetypesofChinesecotton.InvitroCellDevBiol-Plant,2004,40:371-375(SCI)
[24]GUOX,JINS,ZHANGX(2007)Agrobacterium-mediatedtransformationofCry1C,Cry2AandCry9CgenesintoGossypiumhirsutumandplantregeneration.BiologiaPlantarum,51(2):242-248(SCI)
[25]XiyanYang,XianlongZhang,ShuangxiaJin,LiliFu,LinggangWangProductionandcharacterizationofasymmetrichybridsbetweenuplandcottonCoker201(Gossypiumhirsutum)andwildcotton(GossypiumklozschianumAnderss),PlantCellTissureOrganCult,2007,89:225–235(SCI)
[26]YangX,GuoX,ZhangX,NieYandJinS.PlantregenerationfromGossypiumdavidsoniiprotoplastsviasomaticembryogenesis.BIOLOGIAPLANTARUM,51(3):533-537,2007(SCI)
[27]XianlongZhang,ShuangxiaJin,DiqiuLiu,ZhongxuLin,FanchangZeng,LongfuZhu,LiliTu,XiaopingGuo.CottonBiotechnology:ChallengetheFutureforCottonImprovement,In:BiotechnologyinCropImprovement,GPRao(ed.),StudiumPress,LLC,Houstan,Texas,USA.Pp.241-301
[28]LiliTu,XianlongZhang,DiqiuLiu,ShuangxiaJin,JinglinCao,LongfuZhu,FenglinDeng,JiafuTan,CunbinZhang,2007,SuitableinternalcontrolgenesforqRT-PCRnormalizationincottonfiberdevelopmentandsomaticembryogenesis,ChineseScienceBulletin52:3110-3117(SCI)
[29]周小凤,金双侠,李保成,余渝,宁新柱,王旭文.初步研究不同激素对海岛棉体细胞胚胎发生的影响.中国农学通报2009,25(11):78-83
[30]朱华国,涂礼莉,金双侠,徐理,谭家福,邓锋林,张献龙.棉花细胞初始脱分化的基因差异表达分析.科学通报,2008,53(20),2483-492(SCI)
[31]曹景林张献龙金双侠杨细燕朱华国付莉莉.棉花高效体细胞胚发生及同步控制培养体系研究.作物学报,2008,34(2):224−231
[32]谢德意,金双侠,郭小平,张献龙.长江和黄河流域棉区棉花品种体细胞胚胎发生和植株再生能力研究,作物学报,2007,33(3):394-400
[33]谢德意,金双侠,郭小平,张献龙.棉花ipt基因遗传转化效率及优化成苗技术研究,作物学报,2007,33(5)
[34]金双侠,韩杰,刘小云,刘冠泽,王一娴,唐文鑫,张献龙.SDS-蛋白酶法分离棉花cpDNA及psbA基因启动子、终止子克隆.分子植物育种,2007,5,683-689.
[35]周小凤,张碧瑶,刘冠泽,高巍,余渝,邓福军,李保成,孔宪辉,张献龙,金双侠*.新疆棉花高体细胞胚胎发生能力基因型的筛选,分子植物育种,2007,6,1-8(通讯作者).
[36]张献龙,孙玉强,吴家和,金双侠,聂以春,郭小平,2004,棉花细胞工程及新种质创造,棉花学报,16(6):368-373
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