华中农业大学

基本信息姓名: 赵剑 出生年月: 1968.7
性别: 男 硕/博导： 博导
民族: 汉 开设课程： 基础遗传学
职称: 教授 研究方向： 作物代谢组学，大豆作物遗传育种与改良，植物次生代谢和代谢工程，植物特殊营养物和天然药物的生物合成
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学位: 生药学博士

联系方式 办公电话：027-87385199
电子邮件：jianzhao@mail.hzau.edu.cn

个人简介赵 剑， 男，1968年生于内蒙古，博士、教授、博士生导师. 1992年内蒙古师范大学生物系获生物学学士学位，1995年东北师范大学获植物学硕士学位. 1999年中国医学科学院/中国协和医科大学药物研究所获生药学博士学位. 2000-2002年获得日本学术振兴会资助奖励在日本九州大学农学部做抗真菌单萜Thujaplicin的生物合成和生产的研究. 2002-2012年先后在美国堪萨斯州立大学生物化学系、贝勒医学院/美国农业部儿童营养研究中心 （Baylor College of Medicine/USDA/ARS Children’s Nutrition Research Center）和The Samuel Roberts Noble Foundation等大学和研究机构学习和工作。长期从事植物天然化合物的生物合成和代谢工程，脂质代谢等领域的研究。在植物次生代谢和有用天然产物的诱导合成、细胞生物工程生产、生物合成及其调节的分子生物学机理等方面做了大量前沿性研究。近几年来以豆科模式植物Medicago truncatula为研究材料在黄酮类转运和合成调控研究方面，尤其是缩合丹宁和花色素苷的细胞内转运及其合成的转录调节机理方面获得了重大发现并申请了专利，现在其实验室专注于大豆黄酮类化合物，多萜类，油脂等特殊营养物质的生物合成和相关大豆种子品质育种和遗传改良的研究。将豆科模式植物Medicago truncatula 的转录组学和代谢组学研究模式应用到大豆品质的功能基因组学中，用代谢组学和转录组学的技术阐明它们的生物合成和调节的分子机理并把获得的新知识和新手段应用到大豆分子育种和传统育种。

国际学术组织兼职和服务:

从2007年起担任国际期刊 Recent Patents on Biotechnology (by Bentham Science) 的编委; 为The Plant Journal, Plant Physiology, Plant Cell & Environment, Planta, Annals of Botany, New Phytologist, Biotechnology and Bioengineering, Applied Microbiology and Biotechnology; Plant Cell Reports; Biotechnology Progress; Phytochemistry Reviews; Process Biochemistry; Biotechnology and Applied Biochemistry等国际学术期刊的邀请审稿人

科研项目华中农业大学和作物遗传育种与改良国家重点实验室的科研启动费。
科技部973项目—水稻和玉米等作物代谢谱数据库的建立和利用

发明专利及获奖情况主要奖：

2000：日本学术振兴会（Japan Society for the Promotion of Science) 外国人特别研究员奖及研究经费

专利: Verdier JA, Zhao J, Dixon RA, Udvardi MK. Methods and Compositions for Regulating Producing Proanthocyanidins. Pending application to USA patent office.

发表的论文及著作学术专著 (章节):

1. Zhao J, Sakai K. (2001) Involvement of peroxidases and hydrogen peroxide in the metabolism of -thujaplicin in fungal elicitor-treated Cupressus lusitanica suspension cultures. In: Morohoshi N, Komamine A, (eds), Molecular Breeding of Woody Plants: Proceedings of the International Wood Biotechnology Symposium. Progress in Biotechnology, Vol 18, Elsevier: Tokyo, p263-272.
2. Zhao J. (2006) Elicitor signal transduction leading to biosynthesis of plant defensive secondary metabolites. In: Teixeira da Silva J (eds), Floriculture, Ornamental and Plant Biotechnology. Vol 3. Global Science Books Ltd: Tokyo, London. p344-357.
3. Zhao J. (2011) Phytonutrient and phytotherapy for improving health. In: Peter Prendergast and Melvin A. Shiffman (eds), Aesthetic Medicine: Art and Techniques. Springer-Verlag : Heidelberg, Germany, Part 2, p47-58.
4. Zhao J. (2012) Nutraceuticals/functional foods for improving health and preventing disease. In: Oliver Kayser and Heribert Warzecha (eds), Pharmaceutical Biotechnology: Drug Discovery and Clinical Applications, 2nd Edition. Wiley-VCH Verlag: Weinheim, Germany. p599-628

期刊论文(JCR- 2010, Google scholar citation-2015)

Zhao J, Li P, Motes CM, Park S, Hirschi KD. (2015) CHX14 is a plasma membrane K-efflux transporter that regulates K+ redistribution in Arabidopsis thaliana. Plant Cell & Environment in press

Zhao J.(2015) Phospholipase D and phosphatidic acid in plant defence response: from protein–protein and lipid–protein interactions to hormone signaling. Journal of experimental botany. In press
http://jxb.oxfordjournals.org/content/early/2015/02/12/jxb.eru540.full.pdf.

5. Zhao J*, Wang X. (2013) Biochemical analysis of the interaction between phospholipase Dα1 and GTP-binding protein α-subunit from Arabidopsis thaliana. Methods in Molecular Biology 1043:21-35. *通讯作者

6. Zhao J*, Devaiah SP, Wang C, Welti R, Wang X*. (2013) Arabidopsis phospholipase Dβ1 modulates defense responses to bacterial and fungal pathogens. New Phytologist 199:228-240 *共同通讯作者. 杂志影响因子 6.6 被引用次数：16

7. Verdier J#, Zhao J#, Torres-Jerez I, Ge S, He XZ, Mysore KS, Dixon RA, Udvardi MK (2012) The MtPAR MYB transcription factor acts as an on-switch for proanthocyanidin biosynthesis in Medicago truncatula. Proc Natl Acad Sci USA 109:1766-1771 杂志影响因子 9.7； #共同第一作者.被引用次数：34

8. Zhao J, Wang C, Bedair M, Welti R, Sumner LW, Ivan Baxter I, Wang X. (2011) Suppression of phospholipase Dγs confers an increased aluminum resistance in Arabidopsis thaliana. PLoS One 6(12): e28086 杂志影响因子 4.4；被引用次数：15

9. Zhao J, Huhman D, Shadle G, He XZ, Sumner LW, Tang Y, Dixon RA.(2011) MATE2 mediates vacuolar sequestration of glycosylated and malonylated flavonoids in Medicago truncatula. Plant Cell 23: 1536-1555. 杂志影响因子 9.3； 被引用次数：48

10. Zhao J, Dixon RA (2010) The “ins” and “outs” of flavonoid transport. Trends in Plant Science 15:72-80. 杂志影响因子10.0；被引用次数：102

11. Zhao J, Pang YZ, Dixon RA (2010) The mysteries of proanthocyanidin transport and polymerization. Plant Physiology 153: 437-443 杂志影响因子 6.4； 被引用次数：80

12. Zhao J, Connorton JM, Guo YQ, Li X, Shigaki T, Hirschi KD, Pittman JK. (2009) Functional studies of split Arabidopsis Ca2+/H+ exchangers. Journal of Biological Chemistry 284:34075-34083. 杂志影响因子 5.3；被引用次数：30

13. Zhao J, Dixon, RA. (2009) MATE transporters facilitate vacuolar uptake of epicatechin 3'-O-glucoside for proanthocyanidin biosynthesis in Medicago truncatula and Arabidopsis. Plant Cell 21: 2323-2340. 杂志影响因子 9.3； 被引用次数：108

14. Mei H, Cheng NH, Zhao J, Park S, Escareno RA, Pittman JK, Hirschi KD. (2009) Root development under metal stress in Arabidopsis thaliana requires the H+/cation antiporter CAX4. New Phytologist 183:95-105. 杂志影响因子 6.5；被引用次数：28

15. Zhao J, Shigaki T, Mei H, Guo YQ, Cheng NH,, Hirschi KD. (2009) Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. Journal of Biological Chemistry 284: 4605-4615. 杂志影响因子 5.3；被引用次数：28

16. Zhao J, Cheng NH, Motes CM, Blancaflor EB, Moore M, Gonzales N, Padmanaban S, Sze H, Ward JM, Hirschi KD. (2008) AtCHX13 is a plasma membrane K+ transporter. Plant Physiology 148:796-807. 杂志影响因子 6.4；被引用 42次

17. Zhao J, Barkla BJ, Marshall J, Pittman JK, Hirschi KD. (2008) The Arabidopsis cax3 mutants display altered salt tolerance, pH sensitivity and reduced plasma membrane H+-ATPase activity. Planta 227:659-669. 杂志影响因子 3.08；被引用次数：63

18. Mei H, Zhao J, Pittman JK, Lachmansingh J, Park S, Hirschi KD. (2007) In planta regulation of the Arabidopsis Ca2+/H+ antiporter CAX1. Journal of Experimental Botany 58:3419-3427. 杂志影响因子 4.8；被引用次数：43

19. Zhao J. (2007) Interplay among nitric oxide and reactive oxygen species: A complex network determining cell survival or death. Plant Signaling & Behavior 2:544-547 (invited Addendum to New Phytologist 175:215-229). 杂志影响因子 0；被引用次数：17 通讯作者

20. Zhao J. Fujita K, Sakai K. (2007) Reactive oxygen species, nitric oxide, and their interactions play different roles in Cupressus lusitanica cell death and phytoalexin production. New Phytologist 175: 215-229. 杂志影响因子 6.5；被引用次数：30 通讯作者

21. Zhao J. (2007) Nutraceutical, nutritional therapy, phytonutrients, and phytotherapy for improvement of human health: A perspective on plant biotechnology application. Recent Patents on Biotechnology 1:75-97 (invited review). 被引用次数：52 通讯作者

22. Zhao J (2007) Plant troponoids: Chemistry, biological activity, and biosynthesis. Current Medicinal Chemistry 14(24):2597-2621. 杂志影响因子 4.6；被引用次数：28 通讯作者

23. Zhao J, Verpoorte R. (2007) Manipulating indole alkaloid production in Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochemistry Reviews 6:435-457 (Invited review) 杂志影响因子 3.1；被引用次数：73 通讯作者

24. Buseman CM, Tamura P, Sparks AA, Baughman E, Maatta S, Zhao J, Roth MR, Esch SW, Shah J, Williams TD, Welti R. (2006) Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves. Plant Physiology 142:28-39. 杂志影响因子 6.4；被引用次数：115

25. Mishra G, Zhang W, Deng F, Zhao J, Wang X (2006) A bifurcating pathway directs abscisic acid effects on stomatal closure and opening in Arabidopsis. Science 312:264-266. 杂志影响因子 31；被引用次数：283

26. Zhao J, Matsimaga Y, Fujita K, Sakai K. (2006) Metabolic flux and signal analysis of b-thujaplicin and monoterpene biosynthesis in elicited C. lusitanica cell culture by elicitor and methyl jasmonate. Metabolic Engineering 8:14-29杂志影响因子 5.5；被引用次数：17 通讯作者

27. Shigaki T, Barkala B, Miranda-Vergara MC, Zhao J, Pantoja O, Hirschi K (2005). Identification of a critical histidine involved in metal transport activity in Arabidopsis cation/H exchanger CAX1. Journal of Biological Chemistry 280: 30136 - 30142杂志影响因子 5.3；被引用次数：42

28. Zhao J, Fujita K, Sakai K. (2005) Oxidative burst in plant cell culture process: a role in production of　β-thujaplicin by Cupressus lusitanica suspension culture. Biotechnology and Bioengineering 90: 621-631. 杂志影响因子3.7; 被引用次数：38 通讯作者

29. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances 23:283-333. 杂志影响因子5.3；被引用次数：789 通讯作者

30. Zhang W, Qin C, Zhao J, Wang X. (2004) Phospholipase Dα1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling, Proc Natl Acad Sci USA 101:9508-9513. 杂志影响因子9.7；被引用次数：299

31. Zhao J, Zheng SH, Fujita K, Sakai K. (2004) Jasmonate and ethylene signaling pathways and their interaction are integral parts of the elicitor signal transduction leading to phytoalexin biosynthesis in Cupressus lusitanica cell cultures. Journal of Experimental Botany 55:1003-1012. 杂志影响因子4.8；被引用次数：41 通讯作者

32. Zhao J, Guo Y, Kosaihira A, Sakai K. (2004) Rapid metabolism of phosphoinositides and its possible roles in phytoalexin biosynthesis in elicitor-treated Cupressus lusitanica cell cultures. Planta 219:121-131. 杂志影响因子3.0; 被引用次数：24 通讯作者

33. Zhao J, Guo Y, Fujita K, Sakai K. (2004) Involvement of cAMP signaling in elicitor-induced phytoalexin accumulation in Cupressus lusitanica cell cultures. New Phytologist 161:723-733. 杂志影响因子6.5；被引用次数：31

34. Zhao J, Wang X. (2004). Arabidopsis phospholpase D, PLDα1 interacts with Arabidopsis heterotrimeric G-protein α-subunit through a motif analogous to the DRY motif in G-protein-coupled receptors. Journal of Biological Chemistry 279: 1794-1800. 杂志影响因子5.3；被引用次数：131

35. Zhao J, Sakai K. (2003) Multiple signaling pathways mediate fungal elicitor–induced -thujaplicin accumulation in Cupressus lusitanica cell cultures. Journal of Experimental Botany 54:647-656. 杂志影响因子4.8；被引用次数：74

36. Zhao J, Sakai K. (2003) Peroxidases are involved in biosynthesis and metabolism of -thujaplicin in fungal elicitor-treated Cupressus lusitanica suspension cultures. New Phytologist 159:719-731. 杂志影响因子6.5；被引用次数：18

37. Zhao J, Fujita K, Yamada J, Sakai K. (2001) Improved -thujaplicin production in Cupressus lusitanica suspension cultures by fungal elicitor and methyl jasmonate. Applied Microbiology and Biotechnology 55: 301-305. 杂志影响因子3.2; 被引用次数：49

38. Zhao J, Hu Q, Guo YQ, Zhu WH. (2001) Effects of stress factors, bioregulators and precursors on indole alkaloid production in the compact callus clusters culture of Catharanthus roseus. Applied Microbiology and Biotechnology 55: 693-698. 杂志影响因子3.2; 被引用次数：43 通讯作者

39. Zhao J, Hu Q, Guo YQ, Zhu WH. (2001) Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+-influx and the oxidative burst. Plant Science 161:423-431. 杂志影响因子 2.4；被引用次数：73 通讯作者

40. Zhao J, Hu Q, Zhu WH. (2001) Enhanced catharanthine production in Catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors. Enzyme and Microbial Technology 28:673-681. 杂志影响因子 2.9；被引用次数：107 通讯作者

41. Zhao J， Zhu WH，Hu Q (2001) Selection of fungal elicitors to increase indole alkaloid accumulation in Catharanthus roseus suspension cell culture. Enzyme and Microbial Technology 28:666-672.杂志影响因子 2.9；被引用次数：62 通讯作者

42. Zhao J, Wei-Hua Zhu, WH, Hu Q (2001) Effects of light and plant growth regulators on the biosynthesis of vindoline and other indole alkaloids in Catharanthus roseus callus cultures. Plant Growth Regulation 33: 43–49 杂志影响因子 1.6；被引用次数：43 通讯作者

43. Zhao J, Zhu WH, Hu Q (2000) Enhanced ajmalicine production in Catharanthus roseus cell cultures by combined elicitor treatment: from shake-flask to 20-l airlift bioreactor. Biotechnology Letters 22: 509-514. 杂志影响因子 1.7；被引用次数：37 通讯作者

部分中文期刊论文:
1. 赵剑，朱蔚华，吴蕴褀，胡秋 (1999) 反相高效液相色谱法测定长春花组织和细胞培养物中吲哚生物碱的含量。药学学报. 7：
2. 赵剑，朱蔚华，吴蕴褀，胡秋 (1999) 长春花叶片愈伤组织诱导过程中吲哚生物碱的合成模式和含量的变化及相关酶活性的调节。中草药. 1999， 7
3. 赵剑，杨文杰，朱蔚华 (1999) 细胞色素P450和植物的次生代谢。生命科学，1999，2（综合译述）
4. 赵剑，朱蔚华 (1998) 几种红豆杉植物及其愈伤组织的几种同工酶比较研究。中草药. 29（6）：402-405
5. 赵剑，朱蔚华，吴蕴褀 (1998) 红豆杉组织培养中sinenxans高产细胞系TS-19 的同工酶的动态研究。西北植物学报. 18（3）：339-347
6. 赵剑，朱蔚华，方起程 (1997) 紫杉醇及其相关化合物的生物合成和生物工程的研究进展。中草药. 28 （增刊）：28-32（综合译述）<