清华大学

姓　　名：周小红

所在单位：水环境保护教研所

职　　称：副教授

邮寄地址：清华大学中意节能楼424室

电子邮件：xhzhou@mail.tsinghua.edu.cn

教育背景

1998.9-2002.7 同济大学 环境工程 学士

2002.9-2007.7 清华大学 环境工程 博士

工作履历

2013.12-至今 清华大学 环境学院 副教授

2014.2-2014.11 英国南安普顿大学光电中心 访问学者

2009.07-2013.12 清华大学 环境学院 讲师

2008.09-2009.03 加拿大阿尔伯塔大学 访问学者

2007.07-2009.06 清华大学 博士后

教学：

主讲研究生课程：Biofilms: fundamentals to applications(2015-今，32学时)

本科生课程：水中污染物快速检测生物传感器(2017-今，16学时)

本科生课程：环境毒理与健康(2017-今，负责16学时)

研究生课程：现代环境微生物监测原理与技术(2014-今，负责6学时)

学术兼职

Nature期刊Scientific Reports期刊编委(2015-至今)

生物传感、生物芯片与纳米生物技术专业委员会(筹) 委员

国际水协会 会员

中国环境科学学会 高级会员

International Conference on Sensor Device Technologies and Applications (SENSORDEVICES) 国际会议技术方案委员会委员

研究领域

环境监测、环境污染物生物化学分析技术、生物传感器及纳米生物传感器

?研究概况

1. 国家自然科学基金中英交流项目，21711530214，用于环境生物检测的多通道集成光波导芯片，2017/04-2019/03，在研，主持

2. 国家自然科学基金面上基金项目，21677082，基于工程化适配体的水中抗生素快速定量倏逝波传感检测技术，2017/01-2020/12，在研，主持

3. 国家科技部重大仪器开发专项，2012YQ03011101-01，多指标平面波导生物芯片的开发，2012/09-2017/09，在研，参与

4. 国家科技部863计划，2014AA06A506，水质安全生物预警监测技术与系统，2014/01-2017/12，在研，参加

5. 北京市自然科学基金项目，8132032，基于微电极测试的污水深度处理反硝化生物滤池脱氮模型，2013/01-2015/12，已结题，主持

6. 清华大学国际科技合作项目，20133000129，基于功能核酸的重金属检测技术研究，2013/01-2014/12，已结题，主持

7. 国家自然科学基金青年基金，51108243，人工金属氧化物纳米颗粒在污水生物处理系统中的归趋及对微生物聚集体活性的影响，2012/01-2014/12，结题，负责人

8. 微生物聚集体微观测试平台，环境模拟与污染控制国家重点实验室科研仪器设备基金，2012/01-2014/12，结题，主要参加人

9. 国家重大水专项，2011ZX07313-002，污水处理系统区域优化运行及城市面源削减技术研究与示范，2011/07-2015/12，结题，参加人

10. 浙江省自然科学基金一般项目，Y5080009，多元因素影响的水体磷酸盐微传感器信号解析及其芯片设计的研究，2009/01-2010/12，结题，负责人

11. 国家科技部863计划，2009AA06A417，重大环境污染事件特征污染物检测系统与技术集成，2009/08-2012/09，结题，参加

12. 10. 国家重大水专项，2009ZX07313，城市污水处理厂与排水管网优化技术研究与示范，2009/01-2011/12，结题，参加

13. 国家自然科学基金面上项目，50478009，生物载体内部特性的测试与脱氮模型的研究, 2005/01-2007/12，结题，参加

奖励与荣誉

2015年 清华大学环境学院学生工作先进个人

2014年 入选“清华大学桑坦德银行资助项目”

2014年《污水处理在线监测仪器原理与应用(第二版)》获得“2014年中国石油和化学工业优秀出版物奖·图书奖”二等奖(排名第二)

2013年 微量有毒污染物快速高灵敏检测的生物传感技术及仪器，教育部技术发明奖一等奖，排名第六

2011年 教育部“长江学者和创新团队发展计划”创新团队，成员

2010年 第七届中国环境科学学会“优秀青年科技奖”

2009年 入选清华大学骨干人才计划

2009年 “清华大学优秀博士后”称号

学术成果

同行评议SCI期刊论文(*通讯作者)

1. Ruoyu Wang, Xiaohong Zhou*, Bo Liedberg, Xiyu Zhu, Abdul Ghaffar Memon, Hanchang Shi. Screening Criteria for Qualified Antibiotic Targets in Unmodified Gold Nanoparticles-Based Aptasensing, ACS Appl. Mater. Interfaces, 2017, 9, 35492–35497.

2. Xiyu Zhu, Ruoyu Wang, Xiaohong Zhou*, Hanchang Shi. Free-Energy-Driven Lock/Open Assembly-Based Optical DNA Sensor for Cancer-Related microRNA Detection with a Shortened Time-to-Result, ACS Appl. Mater. Interfaces 2017, 9, 25789-25795.

3. Lanhua Liu, Xiaohong Zhou*, Yun Lu*, Didi Shan, Bi Xu, Miao He, Hanchang Shi, Yi Qian. Facile screening of potential xenoestrogens by an estrogen receptor-based reusable optical biosensor. Biosensors and Bioelectronics, 2017, 97: 16-20

4. Lanhua Liu, Xiaohong Zhou*, James S. Wilkinson, Ping Hua*, Baodong Song, Hanchang Shi. Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis. Scientific Reports. 2017, 7: 3655, DOI:10.1038/s41598-017-03939-8

5. Ruoyu Wang, Xiaohong Zhou*, Xiyu Zhu, Chao Yang, Lanhua Liu, Hanchang Shi. Isoelectric bovine serum albumin: Robust blocking agent for enhanced performance in optical-fiber based DNA sensing. ACS Sensors. 2017, 2(2): 257–262

6. Abdul Ghaffar Memon, Xiaohong Zhou*, Jinchuan Liu, Ruoyu Wang, Lanhua Liu, Bofan Yu, Miao He*, Hanchang Shi. Utilization of unmodified gold nanoparticles for label-free detection of mercury (II): Insight into rational design of mercury-specific oligonucleotides. Journal of Hazardous Materials. 2017, 321, 417-423.

7. Lanhua Liu, Xiaohong Zhou*, Mifang Lu, Mingkai Zhang, Chao Yang, Ruiman Ma, Abdul Ghaffar Memon, Hanchang Shi, Yi Qian. An fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples. Sensors and Actuators B: Chemical. 2017, 240, 107-113.

8. Yijun Li, Cheng Wang, Yibo Zhu, Xiaohong Zhou, Yu Xiang, Miao He, Siyu Zeng. Fully integrated grapheme electronic biosensor for label-free detection of lead (II) ion based on G-quadruplex strcture-switching, Biosensors and Bioelectronics, 2017, 89: 758-763

9. Cheng Wang, Yijun Li, Yibo Zhu, Xiaohong Zhou, Qiao Lin, Miao He. High-k solid-gate transistor configured graphene biosensor with fully integrated structure and enhanced sensitivity. Advanced Functional Materials. 2016, 26, 7668–7678

10. Ruoyu Wang, Xiaohong Zhou*, Hanchang Shi, Yi Luo. T-T mismatch-driven biosensor using triple functional DNA-protein conjugates for facile detection of Hg2+. Biosensors and Bioelectronics. 2016, 78, 418-422.

11. Li-pei Zhang, Yun-peng Xing, Lan-hua Liu, Xiao-hong Zhou*, Han-chang Shi*. Fenton reaction-triggered colorimetric detection of phenols in water samples using unmodified gold nanoparticles. Sensors and Actuators B: Chemical, 2016, 225, 593-599.

12. Zhang Jing, Dong Qian, Liu Yanchen, Zhou Xiaohong*, Shi Hanchang. Response to shock load of engineered nanoparticles in an activated sludge treatment system: Insight into microbial community succession. Chemosphere. 2016, 144, 1837-1844.

13. Guo Hongli, Zhou Xiaohong, Zhang Yan, Song Baodong, Zhang Jingxuan, Shi Hanchang. Highly sensitive and simultaneous detection of melamine and aflatoxin M1 in milk products by multiplexed planar waveguide fluorescence immunosensor (MPWFI). Food Chemistry. 2016, 197, 359-366.

14. Han Shitong, Zhou Xiaohong, Tang Yunfei, He Miao, Zhang Xinyu, Shi Hanchang, Xiang Yu. Practical, highly sensitive, and regenerable evanescent-wave biosensor for detection of Hg2+ and Pb2+ in water. Biosensors and Bioelectronics. 2016, 80, 265-272.

15. Gu Chunmei, Long Feng, Zhou Xiaohong, Shi hanchang. Portable detection of Ochratoxin A in red wine based on a structure-switching aptamer using a personal glucometer. RSC Advances, 2016, 6, 29563.

16. Guo Hongli, Zhou Xiaohong, Zhang Yan, Gu Chunmei, Song Baodong, Shi Hanchang. Kinetic analysis of a high-affinity antibody/antigen interaction performed by planar waveguide fluorescence immunosensor. RSC Advances. 2016, 6, 13837.

17. Wang Ruoyu, Zhou Xiaohong*, Shi Hanchang. Triple functional DNA-protein conjugates: Signal probes for Pb2+ using evanescent wave-induced emission. Biosensors and Bioelectronics. 74 (2015) 78-84.

18. Liu Lan-hua, Zhou Xiao-hong*, Shi Han-chang. Portable optical aptasensor for rapid detection of mycotoxin with a reversible ligand-grafted biosensing surface. Biosensors and Bioelectronics. 72 (2015) 300-305.

19. Hao Xiu-juan, Zhou Xiao-hong*, Zhang Yan, Long Feng, Song Lei, Shi Han-chang. Portable and reusable optofluidics-based biosensing platform for ultrasensitive detection of sulfadimidine in dairy products. Sensors, 15 (2015) 8302-8313.

20. Li-pei Zhang, Yun-peng Xing, Chun Liu, Xiao-hong Zhou*, Han-chang Shi*. Label-free Colorimetric Detection of Cu2+ Based on Fenton Reaction Assisted Signal Amplification with Unmodified Gold Nanoparticles as Indicator. Sensors and Actuators B: Chemical, 215 (2015) 561-567.

21. Wang Ruoyu, Xiang Yu, Zhou Xiaohong*, Liu Lanhua, Shi Hanchang. A Reusable Aptamer-based Evanescent Wave All-fiber Biosensor for Highly Sensitive Detection of Ochratoxin A. Biosensors and Bioelectronics. 66 (2015) 11–18.

22. Zhou Xiao-hong*, Huang Bao-cheng, Zhou Tao, Liu Yan-chen, Shi Han-chang. Aggregation behaviour of engineered nanoparticles and their impact on activated sludge in wastewater treatment. Chemosphere. 119 (2015) 568–576.

23. Xing Yun-peng, Liu Chun, Zhou Xiao-hong*, Shi Han-chang. Label-free detection of kanamycin based on a G-quadruplex DNA aptamer-based fluorescent intercalator displacement assay. Scientific Reports. 5(2015) 8125.

24. Xing Yun-peng, Liu Chun, Zhou Xiao-hong*, Zhang Li-pei*, Shi Han-chang. Sensing of Cu2+ Based on Fenton Reaction and Unmodified Gold Nanoparticles. Spectroscopy and Spectral Analysis. (2015) 35(11):3151-3154.

25. Yanchen Liu, Chen Wu, Xiaohong Zhou, Hanchang Shi, D.Z. Zhu. Study on the sulfide elimination by intermittent nitrate dosing in sediments of sewer system. Journal of Environmental Science, 2015, 27(1): 259-265.

26. Yanchen Liu, Qian Dong, Chen Wu, Xiaohong Zhou, Hanchang Shi, Study of the succession of microbial communities for sulfur cycle response to ecological factors change in sediment of sewage system. Environmental science and pollution research international. 01/2015.

27. Liu Lan-hua, Zhou Xiao-hong*, Xu Wei-qi, Song Bao-dong, Shi Han-chang. Highly sensitive detection of sulfadimidine in water and dairy products by means of an evanescent wave optical biosensor. RSC Advances. 2014, 4, 60227–60233.

28. Hao Xiu-juan, Zhou Xiao-hong*, Zhang Yan, Liu Lan-hua, Long Feng, Song Lei*, Shi Han-chang. Melamine detection in dairy products by using a reusable evanescent wave biosensor. Sensors and Actuators B: Chemical, 2014, 204: 682–687.

29. Zhou Xiao-hong1, Song Bao-dong1, Shi Han-chang, Liu Lan-hua, Guo Hong-li, He Miao. An evanescent wave multi-channel immunosensor system for the highly sensitive detection of small analytes in water samples. Sensors and Actuators B: Chemical. 2014, 198: 150-156. (1Contributed equally)

30. Zhou Xiao-hong*, Liu Lan-hua, Xu Wei-qi, Song Bao-dong, Sheng Jian-wu, He Miao, Shi Han-chang*. A reusable evanescent wave immunosensor for highly sensitive detection of bisphenol A in water samples. Scientific reports. 2014, 4: 4572.

31. Song Lei, Zhu Lei, Liu Yan-chen, Zhou Xiao-hong*, Shi Han-chang. A disposable cobalt-based phosphate sensor based on screen printing technology. Science China Chemistry, 2014, 57(9): 1283-1290.

32. Guo Hong-li, Zhou Xiao-hong, Zhang Yan, Shi Han-chang. Highly sensitive and rapid detection of melamine in milk products by planar waveguide fluorescence immunosensor (PWFI). Sensors and Actuators B: Chemical, 2014, 194: 114-119.

33. Zhu Lei, Zhou Xiaohong, Shi Hanchang. A potentiometric cobalt-based phosphate sensor based on screen-printing technology. Frontiers of environmental science & engineering. 2014, 8(6): 945-951.

34. Feng Long, Anna Zhu, Xiaohong Zhou, Hongchen Wang, Zhen Zhao, Lanhua Liu, Hanchang Shi Highly sensitive and selective optofluidics-based immunosensor for rapid assessment of Bisphenol A leaching risk. Biosensors and Bioelectronics, 2014, 55: 19–2524.

35. Shi Han-Chang, Song Bao-Dong, Long Feng, Zhou Xiao-Hong, He Miao, Lv Qing, Yang Hai-Yang. Automated Online Optical Biosensing System for Continuous Real-Time Determination of Microcystin-LR with High Sensitivity and Specificity: Early Warning for Cyanotoxin Risk in Drinking Water Sources. Environmental science & technology, 2013, 47: 4434-41.

36. Xiao-Hong Zhou, Lan-Hua Liu, Xue Bai, Han-Chang Shi. A reduced graphene oxide based biosensor for high-sensitive detection of phenols in water samples. Sensors and Actuators B: Chemical, 2013, 181: 661-667.

37. Xiao-Hong Zhou, Ming-Kai Zhang, Tong Yu, Yan-chen Liu, Han-Chang Shi. Oxygen profiles in biofilms undergoing endogenous respiration. Chemical Engineering Journal, 2013, 220: 452-458.

38. Zhou Xiao-hong, Huang Xin-rui, Liu Lan-hua, Bai Xue, Shi Han-chang. Direct electron transfer reaction of laccase on 1-aminopyrene functionalized reduced graphene oxide. RSC Advances, 2013, 3: 18036-18043.

39. Zhou Xiaohong, Shi Hanchang, Song Yanqing, Wu Yuanyuan. Evaluation of oxygen transfer parameters of fine bubble aeration system in plug flow aeration tank of wastewater treatment plant. Journal of Environmental Sciences, 2013, 25: 295-301.

40. Zhou Xiao-Hong, Wang Xu-Ying, Shi Han-Chang. Inhibitory Effect of Nitrobenzene on Oxygen Demand in Lake Sediments. Journal of Environmental Sciences, 2012, 24(5): 1-6.

41. Liu Jing, Zhou Xiao-Hong*, Shi Han-Chang. Inhibitory effects of pentachlorophenol on wastewater biofilms as determined by phospholipid analysis and microelectrode. Biochemical Engineering Journal, 2012, 66: 8-13.

42. Zhou Xiao-Hong, Liu Jing, Song Hui-Min, Qiu Yu-Qin, Shi Han-Chang. Estimation of Heterotrophic Biokinetic Parameters in Wastewater Biofilms from Oxygen Concentration Profiles by Microelectrode. Environmental Engineering Science, 2012, 29(6): 466-471.

43. Zhou Xiao-Hong, Yu Tong, Shi Han-Chang, Shi Hui-Ming. Temporal and spatial inhibitory effects of zinc and copper on wastewater biofilms from oxygen concentration profiles determined by microelectrodes. Water Research, 2011, 45(2): 953-959.

44. Zhou Xiao-Hong, Qiu Yu-Qin, Shi Han-Chang, Yu Tong, He Miao, Cai Qiang. A new approach to quantify spatial distribution of biofilm kinetic parameters by in situ determination of Oxygen Uptake Rate (OUR). Environmental Science & Technology, 2009, 43: 757-763.

45. Zhou Xiao-Hong, Shi Han-Chang, Cai Qiang, He Miao, Wu Yin-Xi. Function of self-forming dynamic membrane and biokinetic parameters’ determination by microelectrode. Water Research, 2008, 42(10/11): 2369-2376.

46. Zhou Xiao-Hong, Shi Han-Chang*, Qiu Yu-Qin Inner-profiles of a spherical bio-carrier determined by microelectrodes. Biochemical Engineering Journal, 2008, 39(1): 28-36.

47. Zhou Xiao-Hong, Shi Han-Chang, Yu Tong. Characterization of a Spherical Packing Medium for Biological Nitrogen Removal by Microelectrodes. Environmental Engineering Science, 2008, 25(3): 375-382.

参编论著：

1. Chapter 6: An Evanescent Wave Fluorescent Immunosensor for Milk Quality Monitoring in the book of Food biosensor (ISBN: 9781782623618), Xiaohong Zhou, Hanchang Shi. Royal Society of Chemistry, 2016年8月.

2. 污水处理在线监测仪器原理与应用(第二版)[M]. 施汉昌，周小红，刘艳臣. 化学工业出版社，2013年11月.

3. 微生物降解氯苯类化合物机理与应用[M]. 宋蕾，周小红，白润英. 化学工业出版社，2015年3月.

4. 生物接触氧化法污水处理工程技术规范(HJ 2009-2011)，第五编著人.

授权及申报专利：

1. 检测磷酸盐的丝网印刷钴传感器的制备方法(第一/ZL 2008 1 0119649.X)

2. 一种多通道激光诱导荧光芯片(第一/ZL 2015 2 0364595.9)

3. 一种基于核酸适配体探针荧光传感分析检测待测样本中的靶标物质的方法(第一/ZL 201410360025.2)

4. 一种抑制核酸分子对传感界面非特异性吸附的封闭方法(第一/201610509843.3)

5. 一种用BSA等电点封闭液构建可再生DNA杂交界面的方法(第一/201610804155.X)

6. 一种DNA-蛋白质缀合物及其在检测汞离子浓度中的应用(第一/ 201510780448.4)

7. 一种共轴型复合式氧微电极及其制备方法(第一/201510204564.1)

8. 一种检测汞离子的荧光探针及其制备方法(第三/201510498609.0)

9. 一种检测邻苯二酚和/或对苯二酚的探针及方法(第三/201510695541.2)

10. 一种基于T-T错配原理的饮用水中汞离子的荧光检测方法(第三/201510490776.0)

11. 使检测汞离子的生物芯片再生的成套试剂及其应用(第四/201510490778.X)

12. 一种制备核酸芯片的方法及其在倏逝波检测中的应用(第五/201310336822.2)